International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
Bootstrap Slider

Indexed and Abstracted in: Crossref, CAS Abstracts, Publons, CiteFactor, Open J-Gate, ROAD, Indian Citation Index (ICI), Indian Journals Index (IJINDEX), Internet Archive, IP Indexing, Google Scholar, Scientific Indexing Services, Index Copernicus, ResearchBib, Science Central, Revistas Medicas Portuguesas, EBSCO, BOAI, SOROS, NEWJOUR, ResearchGATE, Ulrich's Periodicals Directory, DocStoc, PdfCast, getCITED, SkyDrive, Citebase, e-Print, WorldCat (World's largest network of library content and services), Electronic Journals Library by University Library of Regensburg, SciPeople.

Search Articles

Track manuscript

Advertisement

Full Html

IJCRR - Vol 04 Issue 24, December, 2012

Pages: 07-16

Date of Publication: 30-Nov--0001


Print Article   Download XML  Download PDF

ANTIFUNGAL ANTIBIOTIC PRODUCTION BY STREPTOMYCES SP. ISOLATED FROM SOIL

Author: Shipra Singh, Anita Rawat, Deepak Chand Sharma

Category: General Sciences

Abstract:19 Actinomycetes isolate were obtained from soils samples collected from various ecological niches of Western Uttar Pradesh (Meerut). They were selected for their antimicrobial producing capabilities against some selected microbial strains including fungus (Aspergillus sp.), Yeast (Candida sp.), gram-stain-positive bacteria (Bacillus sp.) and gram-stain-negative bacteria (Burkholderia sp.). Out of 19 isolates, 12 (63%) were found to have antimicrobial activity, among them 53% and 26% isolates were found active against gram-stain-positive and gram-stain-negative bacteria, respectively. Antifungal activity was recorded in 42% isolates, among them 32% were active against Aspergillus sp. and 11% were found active against Candida sp. Only one isolate (SS12) was found to produce broad spectrum antibiotic effective against all test microorganisms. 16S rDNA sequencing and BIOLOG analysis of SS12 suggested that it may be a novel species of genus Streptomyces.

Keywords: Antifungal antibiotic, BIOLOG, 16S rDNA, Streptomyces, Actinomycetes

Full Text:

INTRODUCTION
Actinomycetes, group of heterotrophic, hyphae forming, gram-stain-positive bacteria identified as one of the major group of soil population (Kuster, 1964). These prokaryotes have diverse metabolic requirements and have been explored and exploited for ages in search of various primary and secondary metabolites, such as antibiotic, enzymes and immune modulators (Moncheva et al., 2002). Approximately 70-80% of the antibiotic market is attributed to Actinomycetes; among them more than 50% is contributed by the genera Streptomyces and Micromonospora (Pandey et al., 2004). According to World Health Organization over prescription, excessive and improper use of antibiotics has resulted in the development of Multiple Drug Resistant (MDR) strains of pathogenic microbes. The prevalence of antimicrobial resistance among key microbial pathogens is increasing at an alarming rate worldwide (Singer et al., 2003). This strike back of pathogens is increasing globally and may render the current antimicrobial agents insufficient to control at least some microbial infections (Walsh et al., 2011). This makes the search of novel antimicrobial agents with clinical importance more significant to counter drug resistant microbes. In the present study we have focused on isolation of Actinomycetes from terrestrial soils in ecological niches and check their potential to produce antimicrobials.

MATERIAL AND METHODS
Collection of soil samples Several diverse habitats were selected for the isolation of Actinomycetes. These habitats include cow barns, garden soil, medical waste dumps, garbage dumps etc. Soil samples collected from at least 4 different places at each site. The samples were taken from a depth of 10- 15 cm after removing top soil (Ogunmwonyi et al., 2008), placed in polyethylene bags, closed tightly and stored in a refrigerator at 4°C and processed with in 24h. Test microorganism The test microorganisms used to evaluate the antimicrobial property of isolates were obtained from Subharti medical college, Meerut. The strains represent the various groups of microbes like Aspergillus sp. (Mycellial form of fungi), Candida sp. (Budding form of fungi), Bacillus sp. (gram-stain-positive) and Burkholderia sp. (gram-stain-negative). Isolation of Actinomycetes 1g of soil sample was mixed aseptically in 100mL of sterilized normal saline, and maintained at 50-55 ºC for 1h followed by spreading on petri plates containing Actinomycetes Isolation Agar (AIA) and plates were incubation at 28°C for 10 days (El-Nakeeb and Lechevalier, 1963; Kuster and Williams, 1964). The colonies showing typical morphology (Williams and Cross, 1971) were purified and stored at 4°C in agar slants and as glycerol stock at -20°C. The pretreatment with heat enhances the population of Actinomycetes in soil sample. Screening of Actinomycetes for antimicrobial activity Dual plate assay method The center of PDA plates was point inoculated with test fungi and antagonist bacterial cultures were point-inoculated at the periphery of the plate. Each plate was incubated at 28?C, for 72- 96 hours in an inverted position (Huang et al., 1976). The zone of inhibition of the fungus around each isolate was measured. Identification of the potent Actinomycetes isolate The isolated cultures (Actinomycetes) were classified on the basis of its phenotypic, morphological and biochemical characters. Morphological and microscopic characterization Morphological and cultural characters of the selected actinomycetes strains were studied by inoculating the selected strain into sterile ISP media. The media were sterilized and poured into sterile Petri dish. After solidification of the media, the culture of the selected strain was streaked on the media surface aseptically and incubated at 28 °C for 7 days. Morphological properties such as colony characteristics, type of areal hyphae, aerial mass colour, growth of vegetative hyphae, reverse side pigments, melanoid pigments, fragmentation pattern, spore formation and spore chain morphology were observed (Shirling and Gottileb, 1966).A smear of the selected strain was prepared on a clean glass slide and after performing Gram’s staining was examined under oil immersion (100 X) (Williams, 1993). Biochemical characterization of isolates The isolate was biochemically characterized by sending the cultures to BTK biosciences, New Delhi for Biolog® system analysis (Biolog, USA). Molecular Identification of the strain SS12 DNA isolation of SS12 10 mL of nutrient broth was inoculated with the bacterial isolate and incubated at 30ºC in shaker at 250 rpm. After 12 h, 100µL of glycine (0.1%) solution was added to broth culture and incubated further for 6 h. Genomic DNA was extracted accoeding to Bazzicalupo and Fani (1994). Extracted genomic DNA was electrophoresed on 0.8% agarose gel in TRIS-acetate-EDTA (TAE) buffer (1X) at 80 V for 60 min. Ethidium bromide (0.5 g mL-1 ) was added at the time of gel casting. After the run, gel was visualized under UV transilluminator. Amplification of 16S rDNA 16S rDNA regions of bacterial isolates were amplified from bacterial genomic DNA by polymerase chain reaction, using eubacterial universal primers: Gm3f 5 AGA GTT TGA TCM TGG 3 (8 to 23) Gm4r 5 TAC CTT GTT ACG ACT T 3 (1492 to 1507) Reaction was set in 50µL volume with sterile triple distilled water, Taq DNA Pol. and MgCl2 15 mM (1X), dNTP mix (10 mM, 0.25 mM), Primer Gm3f (0.25 M), Primer Gm4r(0.25 M), Taq DNA polymerase (1.0 U),Target DNA (template), 20-100ng. The amplification cycle consisted of initial denaturation step at 95 °C for 7 min followed by 25 consecutive cycles of 60 sec at 94 °C, 60 sec at 51 °C, 60 sec at 72 °C and three touchdown cycles were successively performed at 54, 53 and 52 °C followed by final extension at 72 °C for 10 min. Positive and negative controls were invariably maintained. The PCR product was run on 0.8% agarose gel and visualized under UV. Sequencing of isolate SS12 The 16S rDNA sequence of the bacterial isolates was determined by dideoxy chain termination method by sending the samples to Chromus India Pvt. Ltd. (Banglore, India). Analysis of sequence data and identification of the bacterium Blast database of the National Centre for Biotechnology information (NCBI) was used to compare the sequences of isolates with known 16S sequences in the existing database. The results obtained were also confirmed by comparing with Ribosomal Database Project. Sequences were aligned through multiple sequence alignment, ClustalW programme, from the European Bioinformatics Institute (EBI) accessible on net (http://www.ebi.ac.uk/clustalW) and phylogram was constructed to understand evolutionary relatedness using MEGA 3.0 (Kumar et al. 2001). Nucleotide sequence accession numbers The 16S rDNA nucleotide sequence obtained in the study was submitted to the GenBank database. RESULT AND DISCUSSION Screening of soil sample Soil samples were collected from eight different locations in Meerut. Emphasis was given to collect soil from places where industrial wastes and household garbage were being dumped. Total 31 actinomycetes strains with varying morphological characteristics were picked up and their pure culture was maintained at 4°C for further studies. Pertinent details of the soil samples and the actinomycetes are shown in Table 1. Soil is known as reach source of Actinomycetes having antifungal activity against plant fungal-pathogens, 110 isolates were screened by Aghighi et al. (2004), from which 14 isolates were found active against various fungal isolates. Screening of isolates for antimicrobial activity Dual plate assay The antimicrobial spectrum of the actinomycetes isolates was confirmed by growing the actinomycetes isolate with the test cultures (Aspergillus sp., Candida sp., Bacillus sp. and Burkholderia sp.). Out of 19 isolates 12 (63%) showed antimicrobial activity against at least one test organism. 53% isolates were found active against at least one gram-stain-positive bacteria and 26% active against at least one gram-stainnegative bacteria and only 32% isolates were active against the fungal test microorganism. Onlyten (53%) isolates were effective against Bacillus sp., five (26%) against Burkholderia sp., two (11%) against Candida sp. and only six (32%) were found to be active against Aspergillus sp. Only one isolate was found effective against all the microorganisms tested (Fig. 1 and Table 2). In a similar study (Oskay, 2004), 50 isolates of actinomycetes were isolated from 10 farming soil samples collected in Manisa Province, Turkey. Approximately 34% of the isolates produced broad and narrow spectrum antibiotics, 16% of the isolates produced antibacterial substance that were active against only gram-stain-positive bacteria 6% of the isolates were active against gram-stain-negative bacteria and 12 % of the isolates were active against both gram-stain-positive and gram-stain-negative bacteria. SS12 was selected for further study due to larger zone size and broad spectrum of activity. Morphological and cultural characteristics of strain SS12 Microscopic observation (1000X magnification) after Gram’s staining revealed that SS12 is a Gram-stain-positive and rod-shaped microorganism (Hucker and Conn, 1923). Other morphological characteristics such as colony characteristics, type of areal hyphae, aerial mass colour, and growth of vegetative hyphae, reverse side pigments, melanoid Pigments, fragmentation pattern and spore formation are detailed in Table 3. They indicate that strains SS12 belongs to the genus, Actinomycetes (Fig. 2) (Shirling and Gottlieb, 1966). Biochemical characterization of isolates Biochemical properties of isolate did not matched with the available data of BIOLOG. The suggested match didn’t belong to actinomycetes; two major possibilities were worked out including novel species or contamination. Second possibility was rejected by the fact that positive and negative control was normal (Fig 3, Table 4). Identification of actinomycetes by biochemical properties are of prime interest (Moncheva et al., 2002) Analysis of sequencing data and identification of the isolates The band of genomic DNA was observed near well and PCR amplicon had a molecular weight of 1.5kb (Fig 4b). The 16S rDNA of the isolate was sequenced, and the sequence of the isolate is shown in Fig 4c. The sequence of 16S rDNA obtained was searched for homogenous sequences in the GenBank database. The results obtained were confirmed by NCBI. This bacterial strain was identified as the member of the genus Streptomyces (BankIt1564851). The isolate did not showed similarity with known isolates of the database. As shown by similarity tree constructed by ClustalW2, and designated as Streptomyces sp. SS12 (Fig. 5). CONCLUSION Nineteen isolates from terrestrial soil of ecologically stressed niches were isolated and their antimicrobial activity against bacteria and fungi was studied. One isolate was found to be the producer of broad spectrum antibiotic and its morphological, cultural and molecular identification indicate that the isolate belongs to genus Streptomyces. Further studies to optimize the culture parameter for economic production of antifungal antibiotic resulted in the enhancement of production level.

References:

1. Aghighi S,  Bonjar GHS, Rawashdeh R, Batayneh S and Saadoun I. First report of antifungal spectra of activity of Iranian Actinomycetes strains against Alternaria solani, Alternaria alternate, Fusarium solani, Phytophthora megasperma, Verticillium dahlia and Saccharomyces cerevisiae. Asian J Plant Sci 2004; 3(4): 463-471.

2. Bazzicalupo M, and Fani R. The use of RAPD for generating specific DNA probes for microorganisms, in Methods in Molecular Biology (Clapp, J. P., ed.), Humana, Totowa, NJ, 1995; 155–175.

3. El-Nakeeb MA and HA Lechevalier. Selective isolation of aerobic actinomycetes. Appl Microbiol 1963; 11: 75-77.

4. Huang HC and Hoes JA. Penetration and infection of Sclerotinia sclerotiorum by Coniothyrium minitans. Can J Bot 1976; 54: 406-410.

5. Hucker GJ and Conn HJ. Methods of Gram staining. Technical Bulletin of the New York State, Agric Exp Station, 1923; p. 93.

6. Kumar, Tamura SK, Jakobsen IB, and Nei M. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 2001; 17:1244-1245.

7. Kuster E, and Williams ST. Selection of media for isolation of streptomycetes. Nature (London) 1964; 202:928- 929.

8. Moncheva P, Tishkov S, Dimitrova N, Chipeva V, Antonova-Nikolova S and Bogatzevska N. Characteristics of soil Actinomycetes from Antarctica. In: Journal of culture collections 2002;3: 03-14.

9. Moncheva P, Tishkov S, Dimitrova N, Chipeva V, Stefka AN, and Bogatzevska N. Characteristics of soil actinomycetes from Antarctica, Journal of culture collections 2002; 3, 3-14.

10. Ogunmwonyi IN, Igbinosa OE, Aiyegoro OA and Odjadjare EE,. Microbial analysis of different top soil samples of selected site in ObafemiAwolowo University, Nigeria Scientific Research and Essay 2008; 3 (3): 120-124.

11. Oskay MA, Usame T, and Cem A,. Antibacterial activity of some actinomycetes isolated form farming soils of Turkey. Afr J Biotechnol 2004; 3(9): 441-446.

12. Pandey B, Ghimirel P, Prasad V, Thomas M, Chan Y, and Ozanick S. Studies of the antimicrobial activity of the actinomycetes isolated from the Khumby region of Nepal. Department of Bacteriology, University of Wisconsin— Madison, Madison, Wisconsin. 2004.

13. Singer RS, Finch R, Wegener HC, Bywater R, Walters J and Lipsitch M. Antibiotic resistance – the interplay between antibiotic use in animals and human beings. Lancet Infect Dis 2003; 3:47-51.

14. Walsh TR, Janis W, David ML and Mark AT. "Dissemination of NDM-1 positive bacteria in the New Delhi environment and its implications for human health: an environmental point prevalence study". The Lancet Infectious Diseases 2011; 11 (5): 355–362.

15. Williams ST and Cross T. Actinomycetes. Applied microbiology. 4, Academic Press; 1971.

16. Williams ST, Locci R, Beswick A, Kurtboke DI, Kuznetsov VD, Lemonnior FJ and Long PF. Detection and identification of novel actinomycetes. Res Microbiol 1993; 144(8):653-656.









Announcements

Dr. Pramod Kumar Manjhi joined Editor-in-Chief since July 2021 onwards

COPE guidelines for Reviewers

SCOPUS indexing: 2014, 2019 to 2021


Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.


Best Article Award

A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19
A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18
A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by situs slot and Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh et al. situs slot "Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma situs slot entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by situs slot et al entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09
Late to bed everyday? You may die early, get depression situs slot
Egg a day tied to lower risk of heart disease situs slot
88 Percent Of Delhi Population Has Vitamin D situs slot  Deficiency: ASSOCHAM Report

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


RSS feed

Indexed and Abstracted in


Antiplagiarism Policy: IJCRR strongly condemn and discourage practice of plagiarism. All received manuscripts have to pass through "Plagiarism Detection Software" test before Toto Macau forwarding for peer review. We consider "Plagiarism is a crime"

IJCRR Code of Conduct: We at IJCRR voluntarily adopt policies on Code of Conduct, and Code of Ethics given by OASPA and COPE. To know about IJCRRs Code of Conduct, Code of Ethics, Artical Retraction policy, Digital Preservation Policy, and Journals Licence policy click here

Disclaimer: International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal.



Company name

International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal

Contact

148, IMSR Building, Ayurvedic Layout,
        Near NIT Complex, Sakkardara,
        Nagpur-24, Maharashtra State, India

editor@ijcrr.com

editor.ijcrr@gmail.com


Copyright © 2022 IJCRR. Specialized online journals by ubijournal .Website by Ubitech solutions

  1. Situs Slot KayaMendadak88
  2. Situs Slot Online Terbaik Dan Terpercaya No 1
  3. Slot Pragmatic Play
  4. Situs Slot Online Terpercaya
  5. Kumpulan Situs Judi Slot Terpercaya
  6. Game Slot Online
  7. Judi Slot Online Jackpot Terbesar
  8. Situs Judi Slot Online Terpercaya 2021
  9. Slot Joker123
  10. Agen Slot Online Resmi
  11. Slot Deposit Pulsa
  12. Daftar Situs Judi Slot Terbaik Dan Terpercaya No 1
  13. AloJudi Slot
  14. KayaMendadak88 Slot
  15. Situs Slot Gacor 2022
  16. Judi Slot Online Jackpot Terbesar Gampang Menang
  17. Joker123 Slot
  18. Daftar Situs Judi Slot Online Deposit Pulsa Terbaik
  19. Mesin slot gacor 88
  20. Mesin slot
  21. Link Slot Online
  22. Slot Via Dana
  23. situs judi slot terbaik dan terpercaya no 1
  24. Daftar Situs Bo Slot Online Gacor Terbaik 2022
  1. Slot Online
  2. Judi Slot Online Jackpot Terbesar
  3. Daftar Situs Judi Slot Online Terpercaya
  4. Judi Slot Terpercaya
  5. Kumpulan Situs Judi QQ Online Terpercaya
  6. Slot Online Terbaru
  7. Situs Game Slot Online Terbaik
  8. Daftar Situs Judi Slot Online Gacor Gampang Menang 2022
  9. Situs Slot Deposit Pulsa dan Slot Online Terbaik
  10. Slot Hacker
  11. Situs Slot Online Terbaik
  12. Slot Gacor Gampang Menang
  13. Situs Judi Slot Online Gacor Terbaik 2022
  14. Situs Judi Slot Online Jackpot Terbesar
  15. Slot Online Terpercaya
  16. Game Slot Penghasil Uang
  17. Situs Slot Online Terbaik 2021
  18. Situs Slot Online Terbaik dan Terpercaya
  19. Situs Slot Terbaik
  20. Situs Judi Slot Terbaik Dan Terpercaya No 1