19 Actinomycetes isolate were obtained from soils samples collected from various ecological niches of Western Uttar Pradesh (Meerut). They were selected for their antimicrobial producing capabilities against some selected microbial strains including fungus (Aspergillus sp.), Yeast (Candida sp.), gram-stain-positive bacteria (Bacillus sp.) and gram-stain-negative bacteria (Burkholderia sp.). Out of 19 isolates, 12 (63%) were found to have antimicrobial activity, among them 53% and 26% isolates were found active against gram-stain-positive and gram-stain-negative bacteria, respectively. Antifungal activity was recorded in 42% isolates, among them 32% were active against Aspergillus sp. and 11% were found active against Candida sp. Only one isolate (SS12) was found to produce broad spectrum antibiotic effective against all test microorganisms. 16S rDNA sequencing and BIOLOG analysis of SS12 suggested that it may be a novel species of genus Streptomyces.