International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
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IJCRR - Vol 05 Issue 15 , August, 2013

Pages: 01-05

Date of Publication: 17-Aug-2013

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Author: Ancy D., Bopaiah A.K.

Category: General Sciences

Abstract:This work was carried out to study the multiplication and growth of Anthurium bicolour (Agnihothri). Plantlets obtained earlier in the solid medium. The liquid culture method showed better growth and development of plantlets. The plantlets obtained were bigger in size with respect to leaf area and shoot height. The multiplication rate was enhanced due to faster absorption of nutrients. Rooting was also better in the liquid medium when compared to the solid medium. The study confirmed that liquid MS medium supplemented with 4 mg\L- BA and 1mg\L- NAA is found more suitable for the growth and multiplication of Anthurium bicolor plantlets.

Keywords: Anthurium Bicolor (Agnihothri), In vitro, regeneration, plantlets.

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Anthurium andreanum has many hybrids which are cultivated as ornamental and for cut flowers, one among them is Anthurium bicolor variety agnihothri with large brightly coloured spathe. It  has a combination of blood red and grass green colour, Hence it is called bicolor. Though Anthuriums are cultivated through regenerative method, the regeneration capacity was found to be less among some of the hybrids. There is great demand for the planting material of Anthurium bicolor variety agnihothri both by the hobbyists and growers. The present work was initiated keeping in mind the demand and faster method for propagation of plantlets. The culture were initiated with solidified agar medium and an attempt was made to grow them using liquid medium supplemented with various concentration of growth additives (Table-1).The response was found to be good in the liquid medium when compared to the solid medium.


Dry matured seeds were collected from the plant and then wash with running tap water, followed by soaking for 5-8 minutes with a mild liquid detergent (10% v/v) teepol. Again the seed were washed thoroughly with running tap water to remove the detergent trace. Then the seed were surface sterilized by dipping the seed in 0.1% v/v mercuric chloride solution for 10-15 minutes, followed by washing with sterile double distilled water 4 to 5 times inside the laminar air flow. They were again dipped in the hydrogen peroxide for 30 seconds and rinsed with sterile double distilled water, followed the standard procedure .These sterilized seeds were inoculated on the surface of solidified full strength MS (Murashige and Skoog 1962) medium supplement with 2 mg\L-BA and 0.5mg\L-NAA. The seed started germination resulting in multiple shoot formation (fig-2) when the shoots were  subcultured in the MS full strength medium supplement with 4 mg\L-BA, 2mg\L-NAA, Myoinositol-100mg\L, thiamine HCl-4mg\L. The plantlets in the solid media was transferred to the liquid medium with same supplements as in the case of solid medium (fig-3). They were incubated at 25oC ±2 oC and (1599-2000 lux) in the growth room. These regenerated   shoots formed cluster with bases adhere to one other (fig-5).The liquid medium was not agitated on the rotary shaker as it used to be done in earlier reports (Whei-Lan Teng 1997). Some of the regenerated shoots formed roots at the base, in the liquid medium (fig-7).

The individual shoots were transferred to MS rooting medium (fig-6), supplemented with Myoinositol, thiamine, glycine, NAA, sucrose, 2%-activated charcoal and agar (Table-1), later plantlets transferred to greenhouse.

The seed germinated in full strength MS medium supplemented with 2mg\L and o.5mg\L-NAA, in about 150 days of time the germinated seed showed multiple shoots (fig-2).The plantlets obtained were further subcultured in MS solid medium supplement with 4mg\L-BAP  and  2mg\L-NAA. The shoot and roots regenerated in about 60 day of time in the MS liquid medium .Where as in the  MS liquid medium,  of  the same composition as MS solid medium regeneration of plantlets were fast compared to the solid medium. The plantlets was transferred to the root inducing medium supplemented with 1mg\L-NAA, sucrose-3%, activated charcoal-2% (Table-1).

It is usual practice to multiply the anthurium plantlets using solidified medium .In the present work an attempt was made to deviate from the monotonous method of culturing them in solidified MS medium. The result was found very positive. The liquid medium was found to be more suitable than the solidified MS medium. This not only saves time in multiplying them. It also saved the cost of producing healthy plantlets, by not using agar in the medium, which is expensive .One more observation made in the is work was usage of, rotary shaker for liquid culture (Whei-Lan Teng 1997). In the present work rotary shaker was totally avoided. The culture were incubated keeping the media in a stagnant condition like the solid media. This also saves the cost of the production by not using extra power for the culture of anthurium plantlets .When the plantlets obtained were transferred to rooting medium (Tables-1), normal root formation was observed (fig-6). When the plantlets were hardened they grew into normal plants without showing any abnormalities (fig-7). This clearly indicates that, by using liquid medium in the later stages of growth, gives better result than the  solid  medium which is normally used in almost all commercial laboratories.


The above work suggest that liquid medium supplement with 4mg\L-BAP and  2mg\L-NAA was found to be better than MS solid media of the same composition for further regeneration and multiplication of plantlets.


The authors wish to thank Dr. Fr. Daniel Fernandes S.J. for is support and encouragement

Financial disclosure: non Funded research work   


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6-Benzyl adenine (BAP), α-Napthaleneacetic acid (NAA), Indole-3-acetic acid (IAA),   , Murashige and Skoog  medium(MS) , Myoinositol(My.I), Glycine(Gly), Thamine (Thy) ,MS Liquid medium(LD),MS Solid medium(SD),MS Rooting medium(RD).

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Best Article Award

A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma COLAKOGLU entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by Arpita M. et al entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09
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Awardees of COVID-19 Research

Woman Researcher Award

A Study by Neha Garg et al. entitled "Optimization of the Response to nCOVID-19 Pandemic in Pregnant Women – An Urgent Appeal in Indian Scenario" published in Vol 12 issue 09

A Study by Sana Parveen and Shraddha Jain entitled "Pathophysiologic Enigma of COVID-19 Pandemic with Clinical Correlates" published in Vol 12 issue 13

A Study by Rashmi Jain et al. entitled "Current Consensus Review Article on Drugs and Biologics against nCOVID-19 – A Systematic Review" published in Vol 12 issue 09

Emerging Researcher Award

A Study by Madhan Jeyaraman et al. entitled "Vitamin-D: An Immune Shield Against nCOVID-19" published in Vol 12 issue 09

Study by Dheeraj Kumar Chopra et al. entitled "Lipid-Based Solid Dispersions of Azilsartan Medoxomil with Improved Oral Bioavailability: In Vitro and In Vivo Evaluation" published in Vol 12 issue 19

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