Introduction: Adenosine deaminase (ADA) is a useful biochemical parameter for tuberculous pleural effusion. Studies have confirmed diagnostic role of Adenosine deaminase for early diagnosis of pleural tuberculosis in high prevalence countries like India. Objective: To assess the diagnostic level of ADA in tubercular pleural effusion and to determine its Sensitivity, Specificity, Positive and Negative predictive value. Material and Methods: The Retrospective study was carried out on 51 patients suffering from pleural effusion in B.J.M.C. Ahmedabad during May to Aug-2012. Detailed clinical history, physical examination and routine investigation of all patients including ADA estimation by Galanti And Giusti method was done. Results : ADA level in tuberculous pleural effusion ranged from 25-160 U/L with a mean level of 72 U/L and at __ampersandsigngt;60 U/L the Sensitivity-69.2%, Specificity-92%, Positive Predictive Value-90% and Negative Predictive Value -74%. So this was best cut off point for ADA level to confirm diagnosis. Conclusion: ADA was found positive with a mean value of 72 U/L in tuberculous pleural effusion with 69.2% sensitivity and 92% specificity at cut off value of 60 U/L.