Background: Immunization done in childhood period almost guarantees protection from many vaccine preventable diseases. It prevents 2 million deaths per year worldwide and is rightly considered to be __ampersandsignlsquo;overwhelmingly good __ampersandsignlsquo;preventive tool by the scientific community. Uptake of vaccination as per national immunization schedule is dependent not only on provision of health services but also on other factors including knowledge and attitude of mothers. Objectives: 1. To determine immunization coverage of infants according to EPI. 2. To access the knowledge of caretakers of infants aged 12 __ampersandsignndash; 23 months regarding immunization. Materials and Methods: It was a cross-sectional study conducted from October 2016 to December 2016, included 210 caretakers and 210 infants of 12-23 months old selected by applying the 30 __ampersandsigntimes; 7 cluster sampling method in block Gudamalani of district Barmer. Only one infant was included from each caretaker. Results: Out of total 210 subjects, 111 (52.85%) were males and rest 99 (47.15%) females. BCG was administered to 106 (95.49%) of males and 85 (85.85%) of females, DPT III to 83 (74.77%) of males and 70 (70.70%) of females and Measles to 149 (70.96%) of the total subjects. The dropout rates in both the gender was observed maximum in BCG to Measles (23.58 in males and 20.00 in females) followed by DPT __ampersandsignndash; I to DPT __ampersandsignndash; III (16.16 in males to 9.75 in females). Knowledge about the correct age of vaccination for Measles, BCG, DPT, OPV were 26.67%, 21.43%, 18.57% and 12.85% of the study subjects respectively. Fever (54.28%), swelling (74.76%), redness (60.95%) on thigh after DPT emerged as main side effects of vaccination. Measles (86.19%) and Polio (65.24%) were the most commonly heard diseases, among the vaccine preventable diseases. Conclusion: Though the coverage rate for vaccine preventable diseases was good in our study, but still there is a need to increase the immunization coverage among the infants, so that not a single child remains unimmunized. Since the infants are vulnerable to infections towards vaccine preventable diseases (VPDs), it necessitates for collection of the data on the knowledge, and practices of the caretakers with respect to different aspects of