Background: The ossification of transverse acetabular ligament is a rare interesting anatomical variation which converts the acetabular notch into a foramen. The transverse acetabular ligament (TAL) contributes to the stability of the joint. Sometimes the ligament gets ossified which limits the movement of hip joint and also leads to the compression of the nutrient vessel and subsequently result in ischemia of the area supplied by it. Ossification of the ligament as found in the present study may be helpful for the clinicians for differential diagnosis. Literature regarding the incidence, cause and clinical implications of this variation is therefore essential for radiologists, orthopaedicians and surgeons operating in the hip replacement surgery. The presence of ossified transverse acetabular ligament was noted and analyzed statistically. The study throws light on the incidence of the ossification of transverse acetabular ligament and discusses its clinical implications. Materials and Methods: Two hundred (214) dry human hip bones (right- 100 and left- 114) were taken for the study. The presence of ossified TAL was noted by macroscopic examination with naked eye. Results were tabulated and statistical analysis was done. Results: 4.3% of bones showed complete ossification of TAL on the left side hip bones and 14% of bones showed incomplete ossification of TAL on the right side and 11.4% on the left side hip bones. Conclusion: The knowledge of incidence of ossified TAL is essential for surgeons, orthopaedicians in performing the hip replacement surgery. The present study may be helpful for clinicians, radiologists and surgeons for differential diagnosis.