Background: There is much evidence suggesting that nutrition and nutritional factors, especially the trace elements zinc and copper may play a role in the formation of human cataract, a disease that is on the increase due to the growing percentage of elderly persons in the world population. Objectives: The present study was done to estimate levels of antioxidant trace elements zinc and copper in the serum samples of senile mature cataract patients. Methods: This is a case control study carried out in the Department of Biochemistry, PES Institute of Medical Sciences __ampersandsignamp; Research, Kuppam, Chittoor district, Andhra Pradesh. 30 senile mature cataract patients and 30 normal age and sex matched controls were selected for the study. Serum zinc and copper levels were measured in above groups. Results: Serum Zinc levels in senile mature cataract patients was 126.63+78.40 __ampersandsignmu;g/dl and in controls 131.01 + 90.2 __ampersandsignmu;g/dl. serum copper levels in senile mature cataract patients was 85.82 __ampersandsignplusmn; 48.1 __ampersandsignmu;g/dl and in controls it was94.23 __ampersandsignplusmn; 52.4__ampersandsignmu;g/dl. The zinc copper ratio was found to be 1.47 in senile mature cataract patients and 1.39 in controls. There was no significant difference in the zinc and copper levels between the two groups. Conclusions: The present study showed no significant alterations in the levels of zinc and copper in serum samples of senile mature cataract patients. Further studies are required involving a larger study group regarding the nutritional behavior, correlation of antioxidant enzymes with zinc and copper levels and the effect of smoking and alcohol consumption on the levels of zinc and copper in senile mature cataract patients.