This study was aim to evaluate the effect of aqueous fruit extract of Hibiscus sabdariffa Linn on spermatogenesis and sperm of mice. Adult male mice (n=30) included in the present study. The mice were eight weeks old and their average weight was 28__ampersandsignplusmn;3g. Male mice housed in temperature controlled rooms (25__ampersandsigndeg;C) with constant humidity (40-70%) and 12h/12h light/ dark cycle according experimental protocols. Thereafter, the mice were randomly divided into control (n=10) and experimental group (n=20). The control group received daily 8 ml distilled water, group-1 of the experimental group received 50 gm/Kg/BW and group-2 received 100 gm/Kg/BW of aqueous extract of Hibiscus sabdariffa. The experiment extended for 21 days. The results of this study showed that there was an increase in the average body weight in the experimental groups compared with the control group with a significant difference (p__ampersandsignlt;0.05). While the testis weight, sperm counts, sperm motility and viability decreased in the experimental groups compared with the control group with a significant difference (p__ampersandsignlt;0.05). The effect of Hibiscus sabdariffa on Sex hormones the study showed that there was significant different (p__ampersandsigngt; 0.05) in FSH; LH and testosterone hormones when compared with control In study of testis histology showed a significant decrease in the lumina spermatozoa. The study concluded that Hibiscus sabdariffa has adverse effect on spermatogenesis and sperm parameters of mice.