Aim: Presenting a case of Desmoplasticameloblastoma with an attempt to differentiate it from fibro-osseous lesion and ameloblastoma Case Report: In this paper we present a case report of a 50yr male who patient presented with a painless swelling in the lower left jaw since 1year. Discussion: Desmoplasticameloblastoma (DA) was included in the World Health Organization Classification of Head and Neck Tumors (WHO-2005) as a variant of ameloblastoma with specific clinical, histological and radiological features to differentiate it from other entities. Radiological and Cone beam volumetric imaging (CBVI) was performed which showed a mixed lesion suggestive of fibro-osseous lesion whereas Incisional biopsy was suggestive of DA. Hence we have attempted to differentiate the three lesions to avoid further misdiagnosis. Conclusion: Desmoplasticameloblastoma cannot be differentiated based only on clinical or only radiological features. A proper collaboration between the physician, radiologist and pathologist is necessary for a proper diagnosis.