Introduction: Blood (plasma) lactate levels estimated from samples collected from different body sites may vary. Lactic acid is produced by skeletal muscle, and since skeletal muscle mass has been shown to be higher on the dominant upper limb, we hypothesized that blood lactate levels from the __ampersandsignldquo;dominant__ampersandsignrdquo; upper limb will be higher than that of the __ampersandsignldquo;non-dominant__ampersandsignrdquo; side at rest. Hence this study was designed to assess the effect of handedness on blood lactate levels drawn from the right and left upper limb. Material and Method: We compared lactate levels in venous blood samples from the right and left antecubital vein and an arterial sample collected from the right upper limb from 14 men and 6 women. Results and Discussion: We found that blood lactate levels between the right and left upper limb sites were comparable. They were also comparable with the arterial blood lactate levels. These findings were observed in both male and female subjects. Thus we conclude that in our sample of subjects__ampersandsignrsquo;, handedness or site of collection of blood sample had no effect on the lactate levels and handedness was not influenced by the sex of the subjects.