Objectives: Current study was undertaken to determine the clinical utility of enzyme biomarkers like Alkaline phosphatase and Lactate Dehydrogenase as well as antioxidant like uric acid in diagnosis , prognosis and metastasis. Methods: 50 clinically and histopathologically confirmed female breast cancer patients of the age group of 30-65 years served as cases and 50 normal healthy females in the same age group served as controls. The parameters were estimated by standard biochemical methods. Results: Serum Alkaline Phosphatase and Lactate Dehydrogenase levels are significantly higher in cases than controls. Where as uric acid levels are non significantly increased in cases. Conclusions: Significant increase of serum enzymes Alkaline phosphatase and Lactate Dehydrogenase along with simultaneous non significant increase of uric acid was observed in the study. These biomarkers can be utilized in remote areas where smaller labs operate at low cost and unexposed to sophisticated knowhow.