Background: Nurses forms an important health care delivering force in tertiary care hospitals.Positive working environment in hospital has enhancing effect on work efficiency and quality of care provided by nurses. Aim: The aim was to find out perception of nurses on working environment and related problem of tertiarycare hospital in Mumbai. Objectives: To know the perception of nurse on problems related to work load, patient related problems and problems due to lack of resources. Materials and Methods: A work site cross-sectional descriptive study was conducted amongst 202 consented nurses of tertiary care hospital in Mumbai. Data was collected by personal interview. Pre-designed semi-structured standardized interview schedule consisting of questions related to Socio-demographic factors, work load, patient related problems and to lack of resources included. Results:In this study 104/202(51.5%) subjects always perceived work load was too much. 84/202 (41.6 %) subjects had to always compromise quality of work because of work load.47/202 (23.3%) subjects always face difficulty in dealing with serious patient33/202 (16.3%) always face difficulty when patient do not recover for long time.136/202(67.3%) subjects always feel that the staff was inadequate to accomplish work properly.97/202 (48.0%) subjects always feel that equipments and supplies were never adequate. Conclusions: Components of working environment in the present tertiary care hospital play a majorrole interfering with work efficiency of nurses and patient care. Proper training and management support needed to make service successful perceived by nurses.