Plant tissue culture is a basic and fundamental component of plant biotechnology and progress in various fields of biotechnology greatly depends on the improvement of this technique. Nowadays, nanomaterials especially Nano silver (NS) are frequently being used as an antimicrobial agent in different fields of sciences including in vitro propagation of plants. Microbial contamination is one of the most serious problems in plant tissue culture and various techniques are being employed to reduce it. This study was carried out to assay the anti-microbial activities of green synthesized silver nanoparticles in the tissue culture growth mediaIn order to assay the efficacy of NS in controlling microbial contamination in the tissue culture; we first synthesized the AgNPs from the leaf extract of Lemon grass (Cymbopogon citratus). The synthesized AgNPswere characterized by UV-vis spectroscopy (UV-vis), and transmission electron microscopy (TEM). After wards, we used various rate of silver nano particles in MS media (5, 10, 20, 25, 40 ml L-1) and the sterilized explants from C. citratuswere cultured on MS medium and evaluatedafter a week interval upto the 4th week of treatment. Adding nano silver at the rate of 40 ml L-1 to growth media was fully effective to control the bacterial infection when evaluated after four weeks of culturing. The finding of this study indicates that NS has a good potential in eliminating of the bacterial contaminants in plant tissue culture.