Background: Palmaris Longus (PL) is a thin, tendinous superficial flexor muscle of forearm. In non-human primates, it is functionally active but considered as vestigial and showing ethnic variations in humans10. But it is commonly used in different hand and plastic surgeries 4. So clinical tests to detect the presence of tendon are found to be very useful for surgeons. Aim: The present study is done to assess the agenesis of Palmaris Longus, unilateral and bilateral by using simple clinical tests. Method: For this study, 240 medical and nursing students of 1st years(boys and girls) were assessed using standard clinical tests. Those with deformities, injuries were excluded. Result: The study showed unilateral absence in 15% and bilateral absence in 8.33%. Absence was more common on left side but not statistically significant. Absence of tendon was more common in females and found statically significant. Conclusion: the present study correlates with previous findings. Combination of all clinical tests is found to be useful to detect the absence of Palmaris Longus