IJCRRI Journ Cur Res ReInternational Journal of Current Research and ReviewI Journ Cur Res Re2231-21960975-5241Radiance Research Academy722HealthcareASSESSMENT OF STAINING QUALITY OF ROSELLE (hibiscus sabdariffa) ON FORMALINFIXED PARAFFIN-EMBEDDED RENAL TISSUE SECTIONS IbnoufAbd-AlhafeezAbdulRaheemEsamSeedAhmedMohamedDahabDalia11112014)3133This article is copyright of Popeye Publishing, 20092009This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.

Objective: This experimental descriptive study aimed to explore the efficiency and resolution of Hibiscus Sabdariffa (HS) as staining dye for renal histological sections as compared to Hematoxylin-Eosin routine stain. Methods: Paraffin-embedded formalin-fixed tissue sections from kidney were stained by Hibiscus Sabdariffa solution using different concentrations and time durations in room temperature. Results: Best results were obtained by using 5% HS solution for one hour. Bad results were noticed mainly when duration of staining was only 1-2 minutes. Conclusion: Hibiscus Sabdariffa is an efficient natural cheap substitute of eosin in the ordinary stain Hand E (Hematoxylin and Eosin) for histological sections.

Hibiscus Sabdariffa Histological Staining