Objectives: The aim of present study was to evaluate the levels of liver enzymes and lipid abnormalities in patients receiving antiretroviral therapy compared to controls. Lactic acidosis was one of the known complications of ART which is the major cause of discontinuation of treatment. Methods: Siemens Dimension Expand Plus fully automated random access analyzer was used for analyzing different parameters. Results: Mean plasma levels of ALT and AST were more in HIV infectedpatients than control group. These parameters were further increased in HIV infected patients after ART. Mean plasma levels of cholesterol and HDL are lower than control group in HIV infected patients before treatment. Cholesterol levels increased after HAART whereas HDL level decreased. Mean plasma levels of triglycerides, LDL and lactic acid are high in HIV infected patients than control and they are further increased after ART. Conclusion: Serum liver enzymes are significantly increased after ART and may be used as prognostic marker of lactic acidosis. This study helps in diagnosing lactic acidosis which can be treated successfully so as to render patients to adhere to treatment to decrease mortality.