Background: Methanogenic microbiome plays an important role in contributing to global renewable energy resource. Globally, biogas production totally estimates to 1/4th of total consumption of fossil fuel. The bioconversion of organic material by anaerobic digestion process to a valuable energy source has a promising outlook. Though there is lot of research contribution to the biogas technology, the knowledge about the microbiome involved in the bioconversion of plant biomass is limited. Objective: In this study, batch fermenter with P. hysterophorus as mono-substrate and along with co-additives of bovine animal__ampersandsignnbsp;faeces at different ratios is presented in the context of molecular data on the microbial composition. Material and methods: A slightly modified protocol of the kit RKN15 to extract the metagenomic DNA from the microbial community participating in the bioconversion of the mono-substrate P.hysterophorus, P. hysterophorus with cow dung and P. hysterophorus nwith goat dropping was performed. Thus extracted and purified DNA from the microbial consortium was subjected for 16S rRNA analysis by terminal restriction fragment length polymorphism (T-RFLP). Result: The minimum estimate number of bacterial community present among the samples with high methane yield revealed the presence of 18 and 14 different types of microbes correspondingly.