Background: The biofilms have a major medical significance as they decrease the susceptibility to the antimicrobial agents. Furthermore, the proximity of cells within a biofilm can facilitate a plasmid exchange and hence enhance the spread of antimicrobial resistance. Objectives: The present study intends to detect biofilm formation and High Drug Resistance amongst the uropathogens and to correlate between biofilm formation and HDRU. Methods: This cross-sectional study was carried out over a period of two months, including 37 catheterized urinary isolate with symptoms of UTI. Following their identification, these isolates were checked for biofilm formation by three different phenotypic methods which includes tube adherence, Congo red agar method __ampersandsignamp; tissue culture plate method. Antibiotic Susceptibility Test was done by Kirby __ampersandsignndash; Bauer Disk Diffusion method as per CLSI guidelines. Results: Out of total 37 uropathogens isolated from catheterized urine samples, 30 (81.1%) were positive in vitro for biofilm production __ampersandsignamp; 22 (59.5%) isolates were HDRU. Maximum biofilm production was shown by E.coli (50%) , followed by Klebsiella pneumoniae (33.3%).