Bioterrorism has been known to exist since hundreds of years by utilising microorganisms as weapons. The terrorist attack using anthrax in the fall of 2001 in U.S. highlighted this kind of utilisation of microorganisms. To criminally prosecute with the help of forensic evidence the perpetrator is necessary and such attacks of bioterrorism are very few. A comprehensive technological network is necessary to strengthen defence against bio crimes by acquiring knowledge of various fields needs to be developed. One such new field connected microbiology and scientific science is known as Microbial Forensics. It utilizes advanced molecular methods like DNA microarray examination and DNA fingerprinting and so on to relate the wellspring of the causative specialist with a particular individual or gathering by measuring varieties between related strains. Excellent affirmation and quality control models for microbial legal sciences will guarantee very dependable outcomes that will stand up in the official courtroom. The more exact and refined a microbial framework turns into, the more appropriate rules for examinations will be characterized. An incorporated approach towards building up this field of microbial crime scene investigation should be taken after, to meet the difficulties of bioterrorism all the more successfully.