Objective: The aim of this study was to assess the knowledge of Ardabil general dentists about Laser applications in dentistry. Methods and Materials: This is a cross sectional descriptive study that has been done on 87 general dentistis in Ardabil city. Data collected by a questinaire and then analyzed by statistical methods in SPSS.19. Results: Of all dentists, 82(94.3%) pointed that only have heard something about Laser. 21 (24.1%) stated that have studies about Laser. Only 2 persons (2.3%) have practical and clinical usage of this method. 60 people (69%) had willing to participate in training courses. The relationship between gender with dentists safity awareness wasn__ampersandsignrsquo;t significant.There are significant diffrencesin general and specializedknowledge between two gender (p=0.024, p=0.002). Conclusion: Results showed that Ardabil general dentistis have lower awareness about laser, so raising awareness and informing about application of laser is essential.