Introduction: Vulvovaginal candidiasis is the second most common cause of vaginitis after bacterial vaginosis and is diagnosed in 40 % women with vaginal discharge. Incidence of fungal infections has increased dramatically over the past few decades. Epidemiologic data from the past decade reveal a paradigm shift in candida species from albicans to non -albicans candida species. The aim of this study was to determine incidence of vulvovaginal candidiasis, identify the species and test their in vitro susceptibility profile against six antifungal agents __ampersandsignndash; Fluconazole, Itraconazole, Ketoconazole, Clotrimazole, Amphotericin B and Nystatin. Material and Methods: Two high vaginal swabs were collected from patient with complain of vaginal discharge. One sample is processed for direct microscopic examination and other sample is used for culture on Sabourad__ampersandsignrsquo;s dextrose agar and Blood agar. Isolates identified by battery of test and antifungal susceptibility testing of Candida species done by Disk Diffusion method. Results: A total of 122 isolates of candida species were obtained. Candida albicans was found to be the most frequently isolated species 81(66.39%) of the total isolates, followed by C. glabrata 19 (15.65%),C. tropicalis 12(33.61%), C. parapsilosis 6(4.91%), C. krusei 3(2.4%) and C. gullermondii 1(0.8%).Non-albicans candida constituted 41(33.61%).Antifungal susceptibility pattern showed that candida isolates were more sensitive to Nystatin and Amphotericin __ampersandsignndash;B, compared to that of Clotrimazole, Fluconazole and Ketoconazole. 78% C. albicans were sensitive to Fluconazole, 5% were dose dependent susceptible, and 17% were resistant. C. krusei had shown 33% resistance against fluconazole and67% resistance against Clotrimazole. Conclusion: The majority of C. albicans isolates were susceptible to Fluconazole. C. krusei had shown maximum resistance among all the isolates. Identification of Candida to species level and their antifungal susceptibility testing should be done to achieve better clinical results.