The present work was focus on the induction of callus by introduced cotyledon, leaf, stem and shoot explants on MS mediumsupplemented with BAP, KIN, IAA, IBA and 2, 4-D in different concentration and combination. MS medium alone with BAP 1.0mg/l and 1.0 mg/l 2, 4-D induce highest percentage of callus, whereas combination of 2, 4-D alone with 0.5mg/l and 1.0mg/l KIN and combination of BAP 1.0mg/l, 1.5 mg/l, 2.0 mg/l, 2.5 mg/l and 3.0mg/l alone with 0.5 mg/l, 1.0mg/l, 1.5mg/l and 2.0 mg/l IAA and IBA were callus reported. Maximum percentage of callus was reported by leaf explant using 1.0 mg/l 2, 4-D combination with 1.5 mg/l BAP. MS medium supplemented with above PGRs either alone or with different combination and concentration all the above explants of C. reticulata were potential capable to regenerated callus.