Aim: Investigation of an outbreak of Cholera in Sankheda village of Chhotaudepur district, Gujarat Introduction: Diarrheal disease outbreaks are causes of major public health emergencies in India. We investigated such outbreak in Sankheda village of Chhotaudepur district, Gujarat to identify the etiological agent, source of transmission and propose control measures. Methodology: This study was conducted by a rapid response team as an investigation of outbreak to find out the root cause during the emergency response. The information was entered and analyzed anonymously. The study was implemented in collaboration with the district health officials Results and Conclusion: The outbreak was caused by Vibrio cholerae 01 Biotype Ogawa. Cases were localized to clusters of village depending on distribution of contaminated water.