Introduction: Malaria is a major public health problem in sub-Saharan Africa. In Guinea, the disease is widespread throughout the country, particularly in the forest region where it is endemic. The objective of this study was to assess the prevalence of malaria in urban and rural areas of Guinea’s forest region by identifying potential socio-economic factors associated with the onset of the disease. Method: Blood samples of 280 patients suffering from malaria (102 and 178 patients in urban and rural areas, respectively) were collected between early May and late August 2022 across healthcare facilities of the forest Guinea, and analysed in the labora tory of the International Centre for Research on Tropical Infections of Guinea (CIRIT-Guinea) for the presence of Plasmodium using the loop-mediated isothermal amplification (LAMP) technique. A questionnaire was used to record information on socio demographic characteristics of patients such as age, gender, place of residence and occupation. Results: Results showed that 63.6% of malaria cases occurred in rural area, compared with 36.4% in urban area, although the difference was not statistically significant (p = 0.12). This could indicate widespread endemicity of the disease in the region. The absence of any significant difference in the distribution of patients by age group (p = 0.76) and gender (p = 0.47) indicates that control strategies should be comprehensive, without prioritizing certain demographic groups. In terms of occupation, malaria was overrepresented among farmers in rural areas (p = 0.03), probably reflecting ongoing exposure to mosquito breeding sites, particularly irrigated farms and stagnant water. Conclusion: These results highlight the need to develop differentiated approaches according to the people occupation. There is need to provide healthcare facilities with additional medical staff and laboratory equipment to ensure reliable diagnosis and to conduct door-to-door awareness campaigns in rural and urban communities on preventive measures against malaria.