The integration of genomics with biodiversity is promising and reveal valuable information about genome organization and genome evolution. Next Generation Sequencing (NGS) technology generated vast genomic resources like short sequence repeats (SSRs), Single Nucleotide polymorphisms (SNPs), Insertions and deletions (InDels), Copy Number Variations (CNVs), Diversity Array Technology (DArT) markers etc. The use of these genomic resources can accelerate biodiversity assessment, conservation, and restoration efforts by enabling scientists to make well-informed decisions. Bioinformatic analyses will further help finding genes, gene products and their function. The genomic technology helps estimating genetic diversity, identification of adaptive alleles, complex diseases and traits, and also enable species identification by DNA barcoding approaches. The use of genomics with classical genetic rescue programs will enable conservation biologists to protect, preserve and mange ecosystems efficiently.