Introduction: Escherichia coli remains the most common cause of urinary tract infections (UTIs). They account for over 80-90% of all community-acquired and 30-50% of all hospital-acquired UTIs. E. coli strains have been found to belong to evolutionary origins known as phylogenetic groups. In 2000, Clermont classified E. coli strains into four phylogenetic groups using the triplex PCR method. The aim of this study was to identify the distribution of the phylogenetic groups amongst E. colistrains using triplex PCR method and to ascertain the antibiotic resistance profile amongst the phylogenetic groups. Methods: In this study, 108 stored E.coli isolates from Dr. Joshi’s Central Clinical Microbiology Laboratory, Vashi, Navi Mumbai were subjected to phylogenetic grouping by a triplex PCR method. Antimicrobial susceptibility testing (AST) was performed by disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Phenotypic detection of multi-drug resistant strains of E. coli based on AST data was performed. Results: All 108 E. coli isolates were found to be multi-drug resistant (MDR). Phylogenetic group B2 (66.67%) was the most predominant, followed by A(15.74%), B1 (12.96%) and D (3.7%). Antibiotic resistance was mainly detected in phylogenetic group B2 (54%). All E. coliphylogenetic groups (B2, A, B1 and D) showed highest sensitivity to carbapenems (imipenem and meropenem) followed by members of aminoglycosides. Phylogenetic groups B2 and B1 recorded sensitivity to some members of fluoroquinolones and cotrimoxazole which was completely absent in group A and D. Majority of the groups exhibited complete resistance to beta-lactams and cephalosporins. Conclusion: Our findings showed the high prevalence of MDR E.coli isolates, with the dominance of phylogenetic group B2. Phylogenetic groups B2 and B1 and A and D showed similar antibioticresistant profiles.