Introduction: Nanoparticles are being used commercially to enhance the effects on the products for better results. Silver nanoparticles are gaining popularity due to their wide range of applications considering their antimicrobial and antifungal properties. This research work uses a quick and ecological method for formation of green synthesized silver nanoparticles and its application as an anti-dandruff shampoo. Methods: Synthesis of silver nanoparticles from Citrus limon and Allium cepa aqueous peel extracts of 5mM and 10mM concentration. Characterization of the nanoparticles was done by FTIR (Fourier transform infrared spectroscopy), SEM (Scanning electron microscopy) and UV-spectroscopy. The formulation of herbal shampoo was carried out. The physical evaluation and antifungal properties (disc diffusion method) of the formulation were determined. Results: UV-spectroscopy showed peaks at a range of 350-450 nm for both nanoparticles after 24hrs and 1 week. SEM analysis showed particle morphology of spherical shape in a range of 30-100nm for both Lemon and Onion nanoparticles. FTIR identified functional groups present in nanoparticles which showed presence of O–H, C=C and C–Br for Citrus limon nanoparticles and H-O-H bending in Allium cepa nanoparticles along with OH, C-Br and C-O groups. These nanoparticles were used to formulate an anti-fungal herbal shampoo using Meethi, Amla, Reetha and Shikakai extracts and anti-fungal activities were studied against the fungi Candida balantits and Candida albicans. The nanoparticles and nanoparticles incorporated shampoo formulation exhibited significant zone of inhibition stating its effectiveness against dandruff causing fungi. Conclusion: The current study included the formulation of an anti-dandruff shampoo with reduced usage of silver nitrate, thereby less quantity of nanoparticles, hence decreasing the risk of toxicity.