Introduction: Derivative UV spectroscopic method is one of the multicomponent analytical method. The combination of Cli dinium bromide (CLB) and Chlordiazepoxide hydrochloride used for to treat stomach or bowel problems including peptic ulcers, irritable bowel syndrome (IBS), and enterocolitis (inflammation of the colon and small intestines. Aim/Objectives: To develop Derivative UV Spectrophotometric method for simultaneous quantification of Clidinium bromide (CLB) and Chlordiazepoxide hydrochloride (CLDZ) in their combined dosage form. Method: This method utilizes ratio of solvent, which is Methanol: Distilled water (10:90 v/v) which make method economic. The λmax of Clidinium bromide and Chlordiazepoxide hydrochloride selected for analysis was found to be 245.46 nm (at ZCP of CLDZ) and 280.06 nm (at ZCP of CLB) respectively. Result: Linearity was observed in the concentration range of 20-120μg/mL for CLB (r2= 0.9997) and 3-18μg/mL for CLDZ (r2= 0.9997) by first order derivative method. The accuracy was found to be 99.62 to 100.33% and for CLB and 99.16 to 99.84 % for CLDZ. The intraday precision was found to be 0.27 - 0.70 and 0.12 - 0.46 for CLB and CLDZ respectively. The Inter day precision was found to be 0.46 – 0.70 and 0.32 -0.66 for CLB and CLDZ respectively. LOD for CLB and CLDZ was found to be 0.1807μg/ ml and 0.0097μg/mL and LOQ doe CLB And CLDZ was found to be 0.5477 μg/mL and 0.0294 μg/mL respectively. Conclusion: The validation parameters found to be complying with ICH guidelines showing % RSD in the desired range. The use of distilled water as a major solvent makes the method economic as well as ecofriendly. The proposed methods can be successfully applied for the routine analysis of both the drugs in dosage form