Background: Upper gastrointestinal tract is an important site for wide variety of lesions especially malignant tumors. Endoscopy in combination with endoscopic biopsy plays an important role in the diagnosis of upper gastrointestinal tract neoplasms and therefore aids in their early management. Aim: To study the histopathology of upper Gastrointestinal endoscopic biopsies and correlate them with clinical presentation, age, sex and to find the density of Helicobacter pylori in gastritis, gastric ulcers and duodenal ulcers. Methodology: A two and a half year observational study was carried out on 132 upper Gastrointestinal endoscopic biopsies. Endoscopic upper Gastrointestinal biopsies were fixed in 10% formalin overnight before processing. Routine Hematoxylin and Eosin stain and a special stain Giemsa was done to detect H. pylori. Results: Out of total 132 upper Gastrointestinal endoscopic biopsies, 37 were from esophagus, 13 from Gastrointestinal junction, 75 from stomach and 7 from Duodenum. Squamous cell carcinoma was more common in esophagus (89%), adenocarcinoma in Gastrointestinal junction (61.5%) and Gastritis in 56% of patients. Dysphagia was common symptom in patients of Squamous cell carcinoma (100%), epigastric pain in patients of adenocarcinoma (42.9%), dyspepsia in gastritis patients (71.4%). Squamous cell carcinoma, adenocarcinoma and gastritis was more common in the age group of 41-60 years with male predominance (66.7%, 84.6% and 69% respectively). Helicobacter pylori gastritis was present in 32 cases (76.1%) while Helicibacter pylori negative gastritis was present in 10 cases (23.8%). Two duodenal ulcer cases were Helicobacter pylori positive (100%) and one gastric ulcer case was Helicobacter pylori positive (33.3%). Conclusion: The endoscopic biopsy not only permits exact diagnosis of specific entity but also provide an opportunity to see H.__ampersandsignnbsp; pylori status and plans for specific medical or surgical therapy. However histopathological study detects mucosal lesions at an early stage especially atrophy, intestinal metaplasia and dysplasia as to prevent progress of these lesions to invasive cancer.