Introduction: Many environmental niches in nature, such the oceans, rivers, soil, caverns, etc., which are home to millions of microorganisms, typically lack sufficient nutrient concentrations. Oligotrophic bacteria have been found in a variety of aquatic and terrestrial environments, including oceans, agricultural fields, forest soils and even ultrapure water. Marine oligotrophic bac teria have been proven in a few studies to be capable of producing a range of essential industrial enzymes, but their full potential has not yet been tapped. Aims: The current investigation focuses on screening Pseudomonas stutzeri for the production of various hydrolytic enzymes such as amylase, lipase, peroxidase, etc. under oligotrophic conditions as well as investigating its morphological alterations under different conditions of nutrient availability. Methodology: The isolate was grown in 1000-fold diluted nutrient agar in presence of specific substrates for screening of enzymes. The morphological changes in the cell were analyzed by FEG-SEM after growing the organism in diluted as well undiluted media. Results: The acquired isolate demonstrated the capacity to flourish in both nutrient-rich and nutrient-limited environments. Under oligotrophic environment, it demonstrated the capacity to synthesize enzymes like amylase, lipase, esterase, and peroxi dases. The cell volume of the isolate was found to decrease by 47% under nutrient limited oligotrophic condition. Conclusion: Our finding broadens the range of potential future studies that could be conducted to explore the immense rel evance of oligotrophs. P.stutzeri shows the production of various enzymes under oligotrophic conditions and should be studied further to optimize the production of enzymes under oligotrophic conditions.