Introduction: Gastric carcinoma is the fifth most common and fourth fatal cancer in the world. The current TNM staging system together with clinicopathological characteristics are not sufficient to detect prognosis. Expression of CDX2 in gastric mucosa is strongly associated with precancerous lesions and cancer. Aim/Objectives: The aim of the study was to evaluate CDX2 expression in different gastric premalignant lesions including intestinal metaplasia, chronic atrophic gastritis, adenoma / dysplasia and in gastric carcinoma and to evaluate the expression between histopathological types, grade and pTNM stages in gastric carcinoma. Methods: It was a cross sectional observational study conducted at Sir Salimullah Medical College and Mitford Hospital, Dhaka over a period of 2 years. CDX2 expression was examined by immunohistochemical method in 77 formalin fixed-paraffin-embedded surgical specimens of gastric precancerous lesions and gastric cancer. The association between CDX2 expression and gastric precancerous lesion was evaluated. The association between CDX2 expression and gastric cancer including histopathological types, grade and pT and pN stages were evaluated. Association between CDX2 expression and specimen type was also evaluated. Results: The mean age of the patient was 55.6+11.9 years ranging from 33 to 86 years and majority of the cases were male (n=53, 68.8%). Among total 77 cases, there were 60 malignant and 17 precancerous lesions. Majority of the cases were endoscopic biopsy specimens (n=42, 54.5%). CDX2 expression was significantly high in precancerous lesions (p=0.039). Among malignant tumors, significantly high expression was observed in well and moderately differentiated (p=0.001) and in low-grade malignant tumors (p