Introduction: Maternal mortality and infant mortality is now act as a danger issue all over the world and the whole world trying to combat these problems by improving the treatment-seeking behaviour at the time of pregnancy as well as improving the facility for delivery at an institution which is comparatively safe than the home delivery. Aim/Objective: The present study gives more attention to the role of antenatal care (treatment-seeking behaviour during pregnancy) on safe delivery (institutional delivery by the skilled birth attendant (SBA) among Indian mothers aged 15-49 years. Methodology: The entire study depends on secondary data collected from the National Family Health Survey (NFHS)-4 in the year 2015-16 in India which was conducted on 1,89,898 women having the age group of 15-49 years. For the proper depiction of the result, bivariate (Pearson’s Chi-square) and multivariate (BLR model) has been conducted. Results: The bivariate and multivariate analysis stated that there were significant variations in the likelihood of being delivered at an institution by the SBA and BLR model showing that there was a significant role of antenatal care in the safe delivery in India though there were also other determinants for safe delivery, the antenatal care was a key indicator for the safe delivery for both mother and newborn baby. Conclusion: This study concluded that the proper utilization of antenatal care services should be given more importance because ANC services is necessary for safe delivery among the women which helps to reduce delivery complications and risk of new born health vulnerability.