Background: Vitamin D is essential for maintaining the integrity of the skeleton as well as proper functioning of the muscles. Postmenopausal women have a significantly increased risk of developing vitamin D deficiency and pelvic organ prolapse, which begs the question of whether or not these two conditions are connected. Aims and Objectives: To study the association of vitamin D deficiency and pelvic organ prolapse in postmenopausal women. Materials and Methods: This study was done in the Department of OBG along with the help of the Department of Orthopedics, Kamineni Institute of Medical Sciences, Andhra Pradesh. The study was done from Oct 2012 to Oct 2013. The recruitment of 60 women with POP and 60 controls took place over the course of two years in a row, during the sun-deprived winter months of November through April. Result: Our study revealed a significant association between vitamin D levels and POP in postmenopausal women. Conclusion: Highly significant relation between Vit D deficiency and prolapse was observed.