Aim: To isolate and identify Candida albicans and non-albicans Candida from various clinical specimen of Intensive care Unit and to investigate some virulence factors and perform their Anti- fungal susceptibility. Materials and Methods: Overall 32 isolates (12 Candida albicans and 20 non- albicans Candida spp.) were included in this study. Virulence factors were studied. In isolates, egg yolk agar was used for determining phospholipase activity, while sheep and human plasma used to determine coagulase activity, Sabouraud dextrose agar with sheep blood for hemolysin activity. Biofilm formation was detected by the tube adherence method. Results: In both Candida spp., it was found that hemolytic activity were higher next to phospholipases activity. It was also found that Coagulase activity were detected in Candida species found to be higher with sheep plasma compared to human plasma. Biofilm production was found to be less and mostly were from blood culture isolates. Conclusion: Knowledge of these virulence factors is an important tool to understand pathogenesis of candidiasis and in addition will help to explore new antifungal drug targets for improved therapeutic regimens.