Introduction: It is generally accepted that assertiveness and high levels of self-esteem are healthy behavioural qualities for all people to possess. It is well acknowledged that assertiveness is one of the most crucial behavioural characteristics for professional nurses. Aims: The purpose of this study was to determine the levels of assertiveness and self-esteem that are held by nurses that work in a certain hospital Materials and Methods: This research was conducted in a non-experimental setting among Registered Nurses, and a total of sixty of them were chosen through the use of the Simple Random approach. The research was started once approval had been granted by the environment and informed consent had been obtained from the participants. The use of a structured questionnaire allowed for an evaluation of nurses’ assertive behaviour as well as their levels of self-esteem. Statistics, both descriptive and inferential, were applied to the study of and analysis of the data that had been obtained. Result: The findings of this study reveal that the majority of nurses working in hospital settings have a self-esteem that is higher than average, with 60% of them having higher self-esteem than the other 40%, who had an average level of self-esteem. While 63.33 percent of nurses displayed good assertive behaviour and 36.67 percent displayed average assertive behaviour, Conclusion: The study came to the conclusion that the majority of the nurses had a high or medium degree of self-esteem while they were working in their separate settings, and that their behaviour was also found to be forceful.