Introduction: This study is to formulate the Euterpe Oleracea in-situ gel for better treatment of conjunctivitis. The Euterpe Oleracea ophthalmic drug solution can treat the ocular infections effectively. Aim: Formulation and evaluation of Eutepe Oleracea in-situ gel for better treatment of conjunctivitis Methodology: Euterpe Oleracea drug containing in-situ gel prepared by using different polymers like carbopol-940, HPMC K15, HPMC K100, HPMC K4M, as a pH triggered gelling system, these polymers are used to controlled release to increase bioavailability. Result: After the formulation prepared it will undergo evaluation i.e. visual appearance, pH, clarity checking, gel capacity, drug content and rheological studies, in-vitro drug release studies, sterility testing were determined. The prepared in situ gel was clear and transparent, it is having good gelling capacity. Conclusion: The drug content of these formulations was determined to be 98%. Developed formulation was stable, and non-irritant, effective therapeutically it can be release in the form of sustained release.