Introduction. High-intensity and long distances exercises have been shown to induce alterations in lung and peripheral muscle function. Nevertheless, alterations and recovery periods have not been studied in response to the repeated sprint ability (RSA) test (6__ampersandsigntimes;35m). Aim: The purpose of this study was to evaluate the effect of a repeated sprint ability (RSA) test on lung function and lower limb power in young soccer players. Method: Twelve young male soccer players (20.1 __ampersandsignplusmn; 1.8 years old) performed a repeated sprint ability (RSA) test (6__ampersandsigntimes;35m). Spirometric parameters, countermovement jump (CMJ) height, lower limb power, and heart rate (HR) were assessed before the test, immediately after the test, and 3, 6 and 9 minutes post-test. Spirometric parameters were measured in the sitting position with a portable Spirobank G. Countermovement jump height and lower limb power were assessed with a Myotest during the countermovement jump and the fatigue index percentage (%FI) was calculated. Results: Immediately after the repeated sprint ability (RSA) test, significant decreases (P__ampersandsignlt; 0.02, P__ampersandsignlt; 0.009 respectively) were observed in forced vital capacity (FVC) and peak expiratory flow rate (PEFR). Lower limb power during the countermovement jump was significantly reduced immediately after exercise (P__ampersandsignlt; 0.001) and was significantly increased at 3 minutes post-RSA. During the repeated sprint ability test, fatigue index percentage (%FI) was significantly high (32.3%). Conclusion: The repeated sprint ability test induced a decrease in lung function, suggesting airway closure. The muscle power of the lower limbs decreased concomitantly with an increase in fatigue index percentage, suggesting muscle fatigue. However, the alteration in lung and peripheral muscle function was not persistent as these soccer players quickly recovered these functions 3-6 minutes after the repeated sprint ability test, suggesting mild and transient mechanisms.