Introduction: Bacteria produce various extracellular substances with variable properties and applications. Among these microbial products, bacteria possessing plant growth-promoting traits together with biosurfactants may improve plant growth in petroleum-contaminated areas. Aims: The aim of the current study is the potential use of biosurfactant-producing plant growth-promoting rhizobacteria (PGPR) to enhance the physio-chemical traits of maize plants grown under petrol stressed soil. Methodology: For the current study, a strategy has been shaped for plant growth improvement using biosurfactant-producing PGPR. Initially, ten bacteria were taken which were screened for biosurfactant production vis-__ampersandsignagrave;-vis plant growth-promoting attributes. After selecting the most efficient bacteria on the basis of screening results, in vitro plant assay was conducted to observe the ameliorating response of maize plant growth, grown under 1 and 2% of petrol stress (v/w) in terms of physio-chemical traits of plants. Results: Three bacteria were selected as the most potent surfactant producing PGPR i.e., Enterobacter cloacae (A9G), Exiguo bacterium sp. (A13G) and Bacillus pumilus (ALa). These bacterial strains revealed their amphiphilic nature by showing high efficiency for flattening and collapsing properties and degrading ability for hydrocarbons (HC) and showed emulsification capability more than 50%. In alliance with this, these strains exhibited auxin and ammonia production potential and showed zinc solubilizing ability. The dual nature of these bacteria made the work worthy for assessing their potential to enhance plant growth under petrol stress. The results of plant assay imparted a coherent picture of increment in growth and biochemical attributes of treated plants while among selected strains, Bacillus pumilus (ALa) exhibited a tremendous increase in all studied parameters. Conclusion: Current study envisages an eco-friendly biological method to improve plant growth under petrol contaminated conditions by showing biosurfactant production, hydrocarbon degradation and plant growth improving attributes.