Background: Malaria continues to be one of the major public health concerns that India is facing since decades. Early identification and management of malaria prevents its complications and subsequent mortality due to it. Several studies across the globe showed that, misconception of malaria still exists and practices of controlling malaria have been unsatisfactory indicating further need of exploring the knowledge, attitude and practices of malaria among the people of high Annual Parasite Incidence (API) states, district and blocks. Aim: The aim of the study is to assess the levels of knowledge, attitude and practices for prevention of malaria in tribal of Jawhar, Maharashtra. Methodology: A cross sectional community based KAP sample survey was carried out with the help of structured questionnaire. Data was collected by face to face interview by using a structured questionnaire from 140 participants by Systematic Random Sampling. Results: The results show, 24 participants (17.1 %) have a low knowledge level and 94 (67.1%) obtained an average level and 22 (15.7 %) have high level of knowledge on components like symptoms, causes and transmission of malaria. 118 participants (84.3 %) have the average level score for attitude component. 88 (62.9 %) of the participants have an average level, while only 14 (10.0 %) found to have low level score on practices component while 38 (27.1 %) found high level practices score. Knowledge on etiology and symptoms of malaria is found to be optimum level, while knowledge regarding the complications and preventive methods were found low among the participants. Health care system is the major source of knowledge for the tribal community. Practices of sanitation and correct health seeking behavior were found to be optimum but the sampled tribal community were found to be not co-relating its importance of health as a whole. Conclusion: Health education program directed towards community should be emphasized on a timely basis to improve the knowledge, attitude, and practice regarding malaria and its prevention in Tribal Communities of Jawhar. Capacity building among the government health workers on preventive part of malaria and complication management for malaria is the need of the hour time, as they are the major contact point for tribals.