Background: Essential hypertension is associated with cardiovascular autonomic imbalance. Heart Rate Variability (HRV) is a non-invasive tool to monitor the functioning of cardiovascular autonomic nervous system (ANS). Objectives: To compare Heart Rate, Blood Pressure and Heart Rate Variability in normotensive and hypertensive adult males. Material and Methods: The study was conducted on 30 normotensive and 30 hypertensive adult males matched for baseline anthropometric characteristics. ECG was recorded and using HRV software, analysis of time domain measures Mean RRI (RR Interval); RMSSD (square root of the mean of the sum of the squares of differences between adjacent NN intervals) and frequency domain measures, LF (Low Frequency); HF (High Frequency); and LF/ HF (Low Frequency/High Frequency) ratio were analysed. Results: The Heart Rate Variability parameters were significantly reduced in hypertensive subjects as compared to normotensives. Conclusion: Reduced Heart Rate Variability indicates impaired regulation of the cardiovascular autonomic function in the hypertensives.