Introduction: Zinc oxide nanoparticles (ZnO NPs) are metal oxide nanomaterials and are widely used in a variety of sectors due to their peculiar physicochemical properties. In the present work, the zinc oxide nanopowder was first chemically synthesized and then detailed characterization is done by various analytical techniques, such as UV-Vis spectroscopy and Fourier Transform Infra-Red (FTIR). Aim: Studies on Characterization and Optimization Parameters of zinc oxide nanoparticles synthesis Methods: In the current study, nanoparticles of zinc oxide were synthesized by a wet chemical process with the help of precursors zinc nitrate and sodium hydroxide the properties of starch were used as a stabilizing material. The powder form of zinc oxide nanoparticles that appeared white in color was purified through the centrifugation process and was stored in an Eppendorf tube at 40C for further work. Results: Synthesis conditions of zinc oxide nanoparticles were optimized using different parameters are metal ion concentrations (0.1M to 0.8M), contact time variations (0.5hr to 4hr), the effect of pH (4 to 9), and effect of temperatures (55 to 850C). The resultant ZnO NPs powder was characterized by UV-Vis spectroscopy and FTIR analysis. Conclusion: This research work was showed the best results of ZnO NPs synthesis and the further part will continue on antimicro bial activity.