Introduction: The use of Computer-Assisted Learning is an important step to implement recommendations of CPCSEA and recently introduced CBME curriculum in practical Pharmacology. It uses the principles of replacing live animal experiments with animal simulator. Objectives: 1) Assess the impact of Computer Assisted Learning (CAL) as an animal simulator in teaching-learning and assessment of 2nd MBBS students in practical Pharmacology 2) Evaluate feedback responses of these students to CAL Methods: This study was done in the Pharmacology department of a tertiary care hospital. Students of 2nd MBBS 3rd semester, undergoing training in new curriculum of CBME (Competency-Based Medical education) as per the directives of NME (old MCI) and MUHS (Maharashtra University of Medical Sciences), Nasik participated in this study. CALsessions on __ampersandsignldquo;effect of drugs on rabbit eye__ampersandsignrdquo; were conducted using software of Medimation Education Pvt Ltd Mumbai. Pre and post tests were conducted to test the impact of student learning. Feedback questionnaire was filled up by students. Assessment of CAL was done in 1stIA (Internal Assessment) of these students and performance was evaluated from the scores. p-value __ampersandsignlt; 0.5 was considered as significant. Observations and Results: It was observed that the performance of the students improved significantly in post- test. Average score increased from 44.4% to 73.5%. The range of scores (1-5 and 6-10) improved significantly in post- test. A positive feedback about CAL was seen in most of structured and open ended questions. Most of the students felt that was that CAL improved their understanding of concepts due to AV impact. The mean score in 1st Internal Assessment of CAL was 75%. Conclusion: CAL is an interesting, informative and motivating tool for teaching learning and assessment in practical Pharmacol ogy. It is a good replacement for live animal experiments. It helps students to understand and clarify the basic concepts.