Introduction: Epilepsy a neurodegenerative disease causes spontaneous and repetitive seizures. Treatment requires instantaneous pharmacotherapy to prevent further the condition of status epilepticus. Aim: The present study was aimed at developing temperature-sensitive in situ gel for intranasal administration of lamotrigine, an anticonvulsant drug used to treat generalised and partial seizure. Methodology: Pluronic 127, chitosan and __ampersandsignbeta;-glycerophosphate were used for the preparation of gel in varying concentrations by cold method. These systems were characterized for physical properties such as pH, gelling temperature, gelling time, drug content, in vitro drug diffusion studies, ex-vivo drug permeation and histopathological studies. The drug polymer compatibility was studied using Fourier transform infrared spectroscopy. Results: All the prepared formulations gelled immediately below 25 sec at the nasal pH and temperature. Addition of chitosan with Pluronic 127 increases the mucoadhesion and contact time of formulation in nasal cavity. The result of in-vitro drug release study revealed 93% of Lamotrigine and 75.33% of drug release in ex-vivo permeability study from the optimized formulation in about 210 min. Result of histopathological studies suggests the suitability of prepared formulation for intranasal administration. Conclusion: In-situ intranasal gel of lamotrigine prepared by using Pluronic 127 and chitosan demonstrated gelation at body temperature and exhibited satisfactory release of drug from its dosage form. It can be concluded that the prepared formulation has potential for the intranasal administration of lamotrigine in the treatment of epilepsy.