Introduction: Venipuncture is one of the commonest painful stimuli children are exposed during hospitalization. Improper management of pain has long lasting effect on psychosocial behavior of children. Many non-pharmacological methods are useful in minimizing procedural pain. Objective: To assess the effect of distraction in form of animated cartoon film on level of pain among hospitalized children during venipuncture. Methodology: Study was conducted among sixty children between 3-10 years of age by using Quasi-experimental posttest only control group design. FLACC or Wong Baker Scale was used for the assessment of pain during venipuncture as per the age of the child. Animated cartoon film in Arabic or English depending on the child__ampersandsignrsquo;s interest, were used as an intervention. It was started five minutes prior to venipuncture and results were documented immediately after the procedure. Results: Majority (66.6%) of the children were between age group of 3-6 years and had (70%) history of previous experience in venipuncture procedure. Distraction in form of cartoon film during venipuncture had shown statistically significant reduction in level of pain among study group. Conclusion: Acute pain associated with venipuncture is an unavoidable traumatic experience for hospitalized children. If not managed appropriately can lead to a long-lasting effect on the psychosocial behavior of the children. Animated cartoon film is an effective and user-friendly method that is well accepted by children and can be implemented by nursing personnel independently.