Introduction: Early and prompt diagnosis of dengue infection can help in improving the clinical outcome of patients by ensuring early initiation of supportive treatment. Non-structural glycoprotein-1 (NS-1) is a useful biomarker for the early diagnosis of dengue as it is abundantly present in the serum during the early stages of infection. Several rapid diagnostic tests targeting NS-1 antigen are commercially available and many more are being developed. The present study evaluated a newly developed fluorescence immunoassay (FIA), NS-1 QUANTI CARD test, to determine its effectiveness in the early diagnosis of dengue. Methods and Material: A total of 85 serum samples from clinically suspected dengue patients were tested for NS1 antigen by ELISA, FIA and rapid immunochromatography test (ICT). The performance characteristics of FIA and ICT were calculated considering ELISA as the gold standard test. Sensitivity, specificity, predictive values, likelihood ratios and Cohen__ampersandsignrsquo;s Kappa value for the level of agreement were calculated using MedCalc__ampersandsignreg; Statistical Software version 19.6.4. Results: The overall sensitivity, specificity, positive and negative predictive values of FIA and ICT were found to be 96.5%, 96.4%, 93.3%, 98.1% and 65.5%, 83.9%, 67.8%, 82.4%, respectively. There was almost a perfect level of agreement between the ELISA and QUANTI CARD test whereas ELISA and ICT results had a moderate level of agreement. Conclusion: Owing to its shorter turnaround time (TAT), the NS1 Ag QUANTI CARD test can be a better alternative to ELISA for dengue diagnosis in resource-limited settings and critically ill patients for prompt diagnosis.