Introduction: Liquid Chromatography Mass Spectrometry (LC-MS/MS) is an exceedingly sensitive and specific analytical tech nique that can precisely determine the identities and concentration of compounds within our sample. Objective: A selective, sensitive, and rapid bio-analytical technique has been developed and it is validated by using Liquid chromatography __ampersandsignndash; Mass spectrometry (LC-MS/MS) process for the estimation of favipiravir and remdesivir in rat plasma Methods: An isocratic mode using the analytical column of waters symmetry C18 150 mm x 4.6 mm, 3.5 __ampersandsignmicro;m, and the mobile phase acetonitrile and buffer in the ratio of 30+70. The method was validated with a linearity range 2-40 ng/mL of both favipiravir and remdesivir. The % CV values of intraday, interlay precision and accuracy were found to be within the acceptance criteria. The % recovery of favipiravir was 96.5% and remdesivir was 99.6% respectively Results: The validation of the method has been done successfully in parameters like accuracy, linearity, recovery, stability and pharmacokinetic studies in rat plasma using LCMS/MS. Stability of the selected drugs present in all conditions like benchtop, wet extract, auto sampler stability, and freeze thaw. Conclusion: This technique gives better results of precision, accuracy, recovery, precision, pharmacokinetic studies, and stability in the rat plasma. The proposed technique was validated as per the guidelines set by International Conference on Harmonization (ICH).