Introduction: Green synthesis of nano silicon oxide powder has been done using rice husk as raw material, which contains more than 91% of silica oxide and other mineral compositions. Aim: To synthesise pure nano-silica oxide powder and utilize pure silica oxide for the production of wound dressings work as an antibiotic. Materials and Method: Rice husks were burned for three hours in a Muffle furnace at 700__ampersandsigndeg;C, then treated with sodium hydroxide solution using a magnetic stirrer at 100__ampersandsigndeg;C for four hours to extract the silica present in the ash to generate sodium silicate (Na2SiO3 ). The pure silica powder is obtained by the titration method with 5N solution of sulfuric acid purification of nano-silica powder is employed through the refluxing method. Results: X-ray diffraction, energy-dispersive x-ray spectroscopy (EDX), field emission scanning electron microscopy (FE-SEM), and FT-IR techniques were used to characterize nano-silica powder. According to Bragg__ampersandsignrsquo;s equation of X-ray diffraction, nano silicon oxide crystals have orthorhombic amorphous forms with average crystal sizes of 33. 406, 53.135, and 10. 10.362 nm. FE-SEM show the partial size in nano size. FT-IR technic shows that the absorbance band at 1072.46 cm-1 refers to asymmetric vibration Si-O-Si (siloxane group), 808.20 cm-1 refers to Si-O-Si bond symmetric vibration. Conclusion: The formation of pure nano Silica oxide from rice husk ash, has been demonstrated to exhibit antibacterial efficacy against Gram-negative bacteria (Escherichia coli), with a concentration of 62.5 __ampersandsignmicro;g/ml as well as utilizing pure silica oxide for the production of wound dressings work as an antibiotic.