Introduction: Organ transplantation is a boon for end-stage organ failure patients as it provides them with long-term survival and better quality of life. Despite this, in India, there is a shortage of organ donors which forms a hurdle in transplantation. One of the reasons may be the knowledge and attitude of health care professionals which are key elements in facilitating the cadaveric organ donation process. Objectives: The present study was designed to assess and compare the knowledge and attitude regarding organ donation among medical, dental and nursing students. Materials and Methods: It was a cross-sectional questionnaire-based study done between October 2019 and March 2020 among 420 undergraduate students of Tagore Medical, Dental and College of Nursing in the district of Chengalpet, Tamilnadu, India. The KAP questionnaire was developed regarding organ donation. The content validation was done and the reliability of the tool was checked by using Cronbach__ampersandsignrsquo;s Alpha test. This validated self-administered questionnaire was provided to the undergraduate medical, dental and nursing students. Results: Out of 420 participants, 180 students were undergraduate medical students. The majority of the participants were in the age groups of 21 to 22 years (37. 8%) and 17 to 18 years (10.6%). The study on the knowledge of the participants showed that the medical students have more adequate knowledge on organ donation. However, the attitude is concerned, more numbers of medical and dental students showed good attitude compared to nursing students. The overall results showed the students of final year and internship have adequate knowledge and a good attitude. Conclusion: The present study which analysed the knowledge and attitude on organ donation among medical, dental and nursing students has been identified that there is a considerable difference in their knowledge and attitude on organ donation. This may be attributed to the differences in the details about organ donation in their curriculum.