Introduction: Upper gastrointestinal tract disorders are one of the most commonly encountered problems in clinical practice.1Endoscopic procedure is performed for a variety of benign and malignant lesions. The definitive diagnosis of upper gastrointestinal tract disorders rests on histopathological confirmation and is one of the bases for proper management and treatment. Objective: To determine the spectrum of histopathological lesions of the upper gastrointestinal tract. To establish endoscopic biopsy as an effective tool in the proper diagnosis of various upper gastrointestinal tract lesions. Material __ampersandsignamp; Method: A retrospective study of 212 upper gastrointestinal endoscopic biopsies __ampersandsignamp;histopathological assessment was done at the department of pathology, Smt. NHL Municipal Medical College and Sheth VS general hospital from Sep 2018- Feb 2020. Results: Of the total 212 cases of the oesophagus, stomach and duodenal biopsies, 66.98%were non-neoplastic lesions and 33.02 were neoplastic lesions. The most common malignancy was squamous cell carcinoma in the oesophagal biopsy. In stomach biopsy chronic gastritis (37.5%) was the most common lesion. In duodenal biopsy chronic nonspecific duodenitis (75%) was the most common lesion. Conclusion: In our study, the commonest site for upper gastrointestinal tract biopsy was the duodenum (34.38%) with 75% nonneoplastic lesions, followed by oesophagus (33.01%) 31.4% non-neoplastic and 68.57% neoplastic lesions. The least common site for biopsy was the stomach (30.1%).In the stomach, non-neoplastic lesions were more common.