Introduction: The long duration of orthodontic treatment is one of the factors that reduce the desire of patients to undergo orthodontic treatment. Many methods have been devised to accelerate the orthodontic movement, but they are not without their negative effects. The injection of platelet-rich fibrin (I-PRF) is one of the modern methods, whose role is still unknown in accelerating dental movement and reduction in the duration of orthodontic treatment. This study aims to evaluate the effectiveness of injection I- PRF to accelerate alignment and levelling of the upper anterior teeth and reduce the time required for treatment. Material and Methods: The sample consisted of 16 patients (10 females, 6 males)in need of orthodontic treatment and who have crowding at the level of the upper anterior teeth. Patients were divided into two 2 groups: the experimental group would be injected with platelet-rich fibrin (I-PRF) and a control group for which only brackets would be applied. The treatment progress was evaluated at 3-time stages, and the studied variables were the rate of improvement in alignment and levelling and the time taken to complete the treatment. Results: The results of the study showed that platelet-rich fibrin injection I-PRF was ineffective in accelerating the alignment and the levelling process (P__ampersandsigngt;0.05) as well as in reducing the time required for treatment (P __ampersandsigngt;0.05). Conclusion: Injection platelet-rich fibrin I-PRF is ineffective in accelerating orthodontic movement.